Laboratory for Expression Systems and Plant Genome Modification (BIOTRON)

• We have produced vector structures for synthesis of synthesis of vector proteins against flu, rabies as well as the artemisin anti-malaria agent in plant.
• We have produced first transgenic plants of common duckweed, tobacco, wolffia arrhiza and chrysantheme that express these genes.
• Our researchers have studied dynamics of growth of duckweeds with content of crude protein and dry matter in plants depending on composition of cultivation environments. As a result of research we selected a composition of environment that is optimal for cultivation of duckweeds – the optimal concentration of macro- and microelements and sucrose.
• The Laboratory has developed protocols of extraction and partial purification of vaccine proteins containing a ricin B subunit. We have developed buffers that are optimal for extraction of proteins from tobacco and duckweed plant biomass; a protocol has been developed for deep purification of recombinant proteins from admixtures and their concentration using differential fractionation by ammonium sulfate.

Collaborations: Nikitsky Botanical Gardens (Russia), Main Botanical Garden of the Russian Academy of Sciences (Russia), All-Russian Research Institute of Agricultural Biotechnology (Russia), Southern Federal University (Russia): collaborative research