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Contract number
075-15-2022-1129
Time span of the project
2022-2024
Invited researcher
since November 2023 Kurchenko Vladimir Petrovich

As of 01.12.2023

30
Number of staff members
6
scientific publications
1
Objects of intellectual property
General information

Name of the project: 

A study the mechanisms of interaction lactic acid microorganisms, lactose fermenting yeast and biologically active substances during microencapsulation of various fractions of microbiota.

Goals and objectives

Goals of project:

The goal of the project is to study the process of microencapsulation of various fractions of microbiota of starter microflora, interaction of individual representatives of mixed cultures and the influence of biologically active substances, from plant-based raw products and microalgae, on the efficiency of fermentation of food substrates by microencapsulated agents.

Project objective:

  1. To study the trends of mixed fermentation of dairy raw materials, cultures of bacteria and lactose-fermenting yeast.
  2. To provide a substantiation of the choice of starter cultures of microorganisms for microencapsulation.
  3. To provide a substantiation of the choice of polymer materials for the encapsulation of starter cultures of microorganisms.
  4. To develop methods of the extraction of biologically active substances from plant raw materials.
  5. To optimize the parameters of the extraction of biologically active substances from plant raw materials.
  6. To develop methods of microencapsulation of lactic acid bacteria and lactose-fermenting yeast.
  7. To optimize the parameters of methods of electrospinning for microencapsulation of various fractions of microbiota of starter cultures.
  8. To develop methods of the extraction of biologically active substances from algae.
  9. To optimize the parameters of the extraction of biologically active substances from microalgae.
  10. To develop methods of encapsulation of biologically active substances.
  11. To study the patterns of fermentation of lactose-containing substrates by microencapsulated starter cultures of lactic bacteria and yeast.
  12. To develop technologies for the production of immobilized nanostructures with biologically active substances by electrospinning.
  13. To research the influence of biologically active substances on the efficiency of fermentation of dairy raw products by encapsulated lactic acid bacteria cultures and lactose-fermenting yeast.
  14. To research the technologies and of functional-purpose fermented drinks and to study their functional characteristics.
The practical value of the study

Scientific results:

Patterns of dairy raw materials mixed fermentation by cultures of lactic acid bacteria and lactose-fermenting yeast.

Validation of starter cultures choice for microcapsulation.

Recommendations on the use of polymer materials for encapsulation of microorganisms starter cultures and biologically active substances extracts.

Optimized extraction parameters of biologically active substances from plant raw materials and microalgae.

Experimental methods of lactic acid bacteria and lactose-fermenting yeast microencapsulation.

Experimental methods of biologically active substances extracts encapsulation.

Experimental methods for obtaining immobilized nanostructures with biologically active substances by electrospinning.

The regularities of lactose-containing substrates fermentation by microcapsulated starter cultures of lactic acid bacteria and yeast.

The effect of biologically active substances on the efficiency of dairy raw materials fermentation by encapsulated cultures of lactic acid bacteria and lactose-fermenting yeast.

Implementation of research results:

Methods have been developed for the production of encapsulated forms of lactic acid microorganisms, extracts of biologically active substances of plant raw materials (sage, purple coneflower, thyme) and whey proteins hydrolysates using a pilot encapsulator, installed at the laboratory in January 2024.

Development of technology for the production of fermented dairy products enriched with biologically active substances of plant extracts.

Development of a technology for the production of fermented drink from whey enriched with biologically active substances of Chlorella microalgae extracts.

Organizational and infrastructural changes:

Creation and development of the research laboratory of Food and Industrial Biotechnology in the structure of the Faculty of Food Engineering and Biotechnology named after Academician A.G. Khramtsov.

Creation of new jobs for students, PhD students and young scientists.

Education and personnel occupational retraining:

Development and implementation of educational programs for the training of highly qualified personnel (PhD level) in the scientific specialties "Food systems", "Biotechnology of food products and biologically active substances".

Development and implementation of master's degree programs in the areas of "Biotechnology", profile "Biotechnology of functional nutrition products and biologically active substances" and "Food of animal origin", profile (focus) "High-tech production of meat and dairy products of a new generation for effective health saving".

Preparation and defence of two PhD theses and two doctoral theses by the staff of the research laboratory.

Scientific internships by laboratory staff at the Belarusian State University (7 persons) and the University of Ljubljana (1 person).

Cooperation:

  • Belarusian State University (Republic of Belarus)
  • University of Ljubljana (Slovenia)
  • Gaziantep University (Turkey)
  • Kemerovo State University (Kemerovo)
  • Educational Centre of Dairy Industry (Moscow)

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Tatsiana M. Halavach, Vladimir P. Kurchenko, Ekaterina I. Tarun, Alexey V. Yantsevich, Veronika V. Shchur, Vasili G. Tsygankow, Aleksey D. Lodygin, Ivan A. Evdokimov, Natasa Poklar Ulrih
Effect of hydrolysis degree with Alcalase on antioxidant and antigenic properties of whey and colostrum protein hydrolysates / Journal of Agriculture and Food Research, 15, 2024, 100975.
Tatsiana M. Halavach, Vladimir P. Kurchenko, Ekaterina I. Tarun, Roman V. Romanovich, Natalia V. Mushkevich, Alexander D. Kazimirov, Aleksei D. Lodygin, Ivan A. Evdokimov
Chitosan complexes with amino acids and whey peptides: Sensory and antioxidant properties / Foods and Raw Materials, 2024, 12 (1).
V.P. Kurchenko, N.V. Sushinskaya, E.V. Chudnovskaya, E.I. Tarun, P.S. Shabunya, A.D. Lodygin, I.A. Khrustaleva, A.N. Kupriyanov
Variation in Composition of Biologically Active Substances in Flowers and Leaves of Achillea asiatica Serg. Plants Depending on the Region of Habitat / Russian Journal of Plant Physiology, 2023, Vol. 70:174.
Tatsiana M Halavach, Vladimir P Kurchenko, Ekaterina I Tarun, Natalia V Dudchik, Mikalai M Yatskou, Aleksey D Lodygin, Ludmila R Alieva, Ivan A Evdokimov, Natasa Poklar Ulrih
Influence of Complexation with β- and γ-Cyclodextrin on Bioactivity of Whey and Colostrum Peptides / International Journal of Molecular Sciences, 2023, 24(18).
Ntsefong, G.N., Lodygin, A., Evdokimov, I., Oboturova, N., Rzhepakovsky, I., Nersesyan, T., Povetkin, S., Nagdalian, A.
Polymer selection for microencapsulation of probiotics: impact on viability, stability, and delivery in functional foods for improved manufacturing and product development in the food industry / Potravinarstvo Slovak Journal of Food Sciences, 2023, 17.
V.P. Kurchenko, T.N. Golovach, K.I. Maiorоva, M.I. Shramko, A.D. Lodygin, I.A. Evdokimov
Application of chitosan for fermented dairy products with Lactobacillus delbrueckii subsp. Bulgaricus manufacturing / Intelligent Biotechnologies of Natural and Synthetic Biologically Active Substances. Cham. ‒ 2022. ‒ PP. 167-175.
Ilja Gasan Osojnik Cˇrnivec, Tigran Neresyan, Yuliana Gatina, Vid Kolmanicˇ Bucˇar,Mihaela Skrt, Iztok Dogša, Bojana Bogoviˇc Matijaši´c, Irina Kulikova, Aleksei Lodygin, Nataša Poklar Ulrih
Polysaccharide Hydrogels for the Protection of Dairy-Related Microorganisms in Adverse Environmental Conditions / 2021, Vol. 26 (24), 7484
Method of obtaining biomass of microalgae Chlorella vulgaris. Patent RU 2797012 C1. Registration date 30.05.2023
Naghdalyan A.A., Blinov A.V., Abaturova N.P., Golik A.B., Maglakelidze D.G., Yakovenko A.A., Kolodkin M.A.
Other laboratories and scientists
Hosting organization
Field of studies
City
Invited researcher
Time span of the project
Laboratory of the Environmental Problems of Post-industrial Agglomeration

South Ural State University (national research university) - (SUSU)

Environmental and industrial biotechnologies

Chelyabinsk

Stankovic Dalibor Miodrag

Serbia

2022-2024

Laboratory for Molecular Microbiology

Peter the Great St.Petersburg Polytechnic University - (SPbPU)

Environmental and industrial biotechnologies

St. Petersburg

Severinov Konstantin Viktorovich

USA, Russia

2013-2017