Laboratory for Stem Cell Genome Modification

• We have created a bank of E.Coli strains and plasma vectors carrying genetic structures necessary for targeted genome modification.
• Our researchers have prepared lines of the 129Ola embryo stem cells and tested methods of their modification and cloning.
• Optical methods for research of glycocalyx. Data has been obtained on the key role of vessel endothelium glycocalyx in development of atherosclerosis.
• We have refined the method of implanting telemetric sensors for ECG.
• We have refined the method of hypertrophy of vessel walls in response to mechanical damage of endothelium.
• We have created in vitro and in vivo models that allow us to analyze drugs and search for new targets for treatment of the Alzheimer's disease.
• Our researchers have refined a method of immunoferment detection of syndekane in mouse blood plasma. A comparison of a commercial set for determining human syndekane with antibodies to syndekane-1 of mice produced at our Laboratory. We have shown high specificity of the method that allows to determine amount of syndekane diluted in plasma. We have also shown that in the process of development of atherosclerosis in mice of the ApoE line concentration of syndekane increases. We have drawn blood samples from mice with different stages of atheroma development.

Education and career development: 6 masters dissertations have been defended

Collaborations:

University of Edinburgh (United Kingdom), A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences (Russia): joint research