We use cookies.
By using the site, you agree to our Privacy Policy.

Contract number
Time span of the project
Head of the laboratory

As of 01.11.2022

Number of staff members
scientific publications
Objects of intellectual property
General information
Name of the project:  Bioluminescent biotechnologies

Goals and objectives

Project objective: Development of highly efficient analytical bioluminescent technologies and methods of their application in biology, medical diagnostics and monitoring of the state of environment, study of molecular and cellular organization of bioluminescent systems of various organisms

Research directions:

  • Study of molecular and cellular organization of bioluminescent systems of various organisms
  • Development and applications of highly efficient methods of analytical usage of bioluminescence for biology, medical diagnostics, and in monitoring of the state of the environment
  • Creation of educational programs based on the phenomenon of bioluminescence

The practical value of the study

Scientific results:

  • We have uncovered the mystery of the luminescence of fungi and worms, found marker substances that can be used in medical diagnostics. We created bioluminescent diagnostic preparation for detecting socially significant diseases and infections.
  • The Laboratory has studied the main bioluminescence properties of eight new analogs of coelenterazine in reactions catalyzed by Са2+-regulated photoproteins, which is important for the imaging of calcium flows in cells and tissues of small animals by using the «red» shift of the spectrum of bioluminescence.
  • Our researchers have proposed a platform technology for multiplex bioluminescence testing.
  • The Laboratory has developed a technology for producing immobilized multi-component preparations for conducting fermentation analysis in environmental studies and medicine as biological modules of ferment biosensors. We produced an experimental lot of microfluidic chips with reagents placed inside that allow to detect toxic substances at the level of the MPC (maximum permissible concentration). 

Implemented results of research: 

We have organized the production of a commercial product, a portable bioluminescence express-laboratory for the environmental and medical monitoring of toxicity. It consists of the «LumiShot» bioluminometer, the «Enzymolum» reagent and a set of methodologies for determining the toxicity of an medium for medical, environmental and industrial applications, such as the assessment of water, air and soil pollution, the toxicity of nanomaterials and medical-purpose materials, food additives, the degree of gravity of neurotoxicosis, the impact of stress on athletes. «Research and Production Association «Applied biosystems» LLC produces the device and reagents under a license agreement. Since 2010 we have been selling «Enzymolum» laboratories has been sold to education institutions of Russia (universities, high schools and additional training institutions) as the School Environmental Laboratory. 

Education and career development:

  • For employees of the Laboratory internships have been organized at Russian and foreign universities. Bachelor’s and master’s degree students received an academic mobility opportunity to study at the University of Eastern Finland within the FIRST+ program.
  • 8 Candidate of Sciences dissertations, 42 bachelor’s degree theses, 29 master’s degree theses have been prepared and defended (of which 3 master’s degree theses have been defended by foreign students).
  • One foreign scientist works at the Laboratory as a research fellow.
  • Employees of the Laboratory in collaboration with the Department of Biophysics of the Institute of Basic Biology and Biotechnology of Siberian Federal University provide training in the following profiles in Physics: «Biochemical physics» (bachelor’s degree) and «Biophysics» (master’s degree); in Biology: «Biophysics» (bachelor’s degree, network master’s degree in collaboration with Baltic Federal University, postgraduate degree).
  • Employees of the Laboratory in collaboration with the Department of Biophysics of the Institute of Basic Biology and Biotechnology of Siberian Federal University have developed the following master’s degree programs in English: «Biological engineering» and «Medical and Biological Physics». The development of the master’s degree programs in English «Medical and Biological Physics» was supported within a grant competition for master’s degree professors organized by the charity program «Vladimir Potanin’s Fellowship Program» of the Vladimir Potanin Foundation.
  • The following academic events have been organized: the International bioluminescence seminar under the supervision of Professor Osamu Shimomura, Academician Iosif I. Gitelzon (2012, 2013), the working seminar «Bioluminescence biotechnologies» under the supervision of Professor Osamu Shimomura Academician Iosif I. Gitelzon (2012), the «Biophysics» section within the International Research and technology conference for undergraduate and postgraduate students and young researchers «Free Avenue» (2015–2022), Doors open days at the Siberian Federal University (2013-2022), the All-Russian science festival «NAUKA 0+» (2015–2018), the high-intensity residential school of Siberian Federal University «Baby Squirrel» (2015–2019) and «Creative Me» (2017–2018), the International satellite conference «Environmental monitoring. Methods and approaches» (2021), the school for talented children «Future Biotech» (2018–2022). 
  • We have developed a modern laboratory case study for schools in Biology and Environmental studies. The school laboratory complex «Enzymolum» for its implementation has improved the quality of school education in Krasnoyarsk Krai and Khanty–Mansi Autonomous Okrug. 7 scientific laboratories have been created at schools to conduct research in bioluminescence. 

Organizational and structural changes: 

On the grounds of the Laboratory two Small Innovative Enterprises have been organized («Research and Production Association «Applied biosystems» LLC and «Biolumdiagnostika» LLC) to manufacture bioluminometers, reagents for bioluminescence analysis. We have created the professional association of natural scientists «Open Laboratory of Advanced  Research and Technologies» to manufacture and test microfluidic chips. 

Other results:
  • The Laboratory for Bioluminescent Biotechnologies is one of the key laboratories that allowed Siberian Federal University to enter Project 5–100, an initiative to increase the  international competitiveness of Russian universities.
  • In 2015, the Osamu Shimomura Foundation was organized pursuant to a donation contract signed by the leading scientist. Since 2016 we have been organizing annual competitions for fellowships from the Foundation for successful research in bioluminescence among postgraduate and undergraduate and school students. In 2016 , four young researchers received scholarships, 12 young researchers received it in 2017 and three in 2018


  • University of Bologna (Italy), B
  • iomedical Science Institute of Medicine of the University of Tsukuba (Japan), 
  • KTH Royal Institute of Technology (Sweden),
  • MicroBioTests Inc. (Belgium), 
  • Jamia Hamdard University, Central Food Technological Research Institute (India), 
  • University of Cádiz, University of Barcelona (Spain), 
  • Czech Technical University in Prague (Czech Republic), 
  • Friedrich Schiller University Jena (Germany), 
  • University of Cyprus (Cyprus), 
  • University of Southern Denmark (Denmark), 
  • National Research Center (Egypt), 
  • Moscow State University, A. N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, 
  • Tomsk Polytechnic University, 
  • Institute of Protein Research of the Russian Academy of Sciences, 
  • Institute of Cell Biophysics of the Russian Academy of Sciences, 
  • Irkutsk State University, 
  • Immanuel Kant Baltic Federal University (Russia): joint research and publications, academic mobility for master’s degree and postgraduate students, staging and participating in conferences, launching a network master’s degree program.

Hide Show full
.pande shubhra, ranjan rajeev, ryazanova maria, shuvaev anton n., salmina alla b., kratasyuk valentina a.
Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats// Food Chemistry. - 2022, -Vol. 373, part B: 131507.
.deeva aa, lisitsa ae, sukovatyi la, melnik tn, kratasyuk va, nemtseva ev
Structure-Function Relationships in Temperature Effects on Bacterial Luciferases: Nothing Is Perfect. International Journal of Molecular Sciences. 2022; 23(15):8119. https://doi.org/10.3390/ijms23158119
esimbekova e.n., kalyabina v.p., kopylova k.v., torgashina i.g., kratasyuk v.a.
Design of bioluminescent biosensors for assessing contamination of complex matrices // Talanta. – 2021. – V. 233. – P. 122509.
kratasyuk v. a., stepanova l.v., ranjan r., sutormin, o.s., pande s., zhukova g.v., miller o.m., maznyak n.v., kolenchukova o.a.
A noninvasive and qualitative bioluminescent assay for express diagnostics of athletes' responses to physical exertion // Luminescence. – 2021. – V. 36. – №. 2. – P. 384-390.
kratasyuk v. a., kolosova e.m., sutormin o.s., lonshakova-mukina v.i., baygin m.m., rimatskaya n.v., sukovataya i.e., shpedt a.a.
Software for Matching Standard Activity Enzyme Biosensors for Soil Pollution Analysis // Sensors. – 2021. – V. 21. – №. 3. – P. 1017
kalyabina v.p., esimbekova e.n., kopylova k., kratasyuk v.a.
Pesticides: formulants, distribution pathways and effects on human health–a review // Toxicology reports. – 2021. – V. 8. – P. 1179-1192.
lisitsa a.e., sukovatyi l.a., bartsev s.i., deeva a.a., kratasyuk v.a., nemtseva e.v.
Mechanisms of viscous media effects on elementary steps of bacterial bioluminescent reaction // International journal of molecular sciences. – 2021. – V. 22. – P. 8827.
sutormin o. s., sukovataya i. e., pande s., kratasyuk v. a.
Effect of viscosity on efficiency of enzyme catalysis of bacterial luciferase coupled with lactate dehydrogenase and NAD(P)H:FMN-Oxidoreductase // Molecular Catalysis. – 2018. – V. 458. – P. 60-66.
nemtseva elena, gulnov dmitry, gerasimova marina a., sukovatyi lev a., burakova ludmila p., karuzina natalya e., melnik bogdan s., kratasyuk valentina a.
Bacterial luciferases from vibrio harveyi and photobacterium leiognathi demonstrate different conformational stability as detected by time-resolved fluorescence spectroscopy// International Journal of Molecular Sciences- 2021, 22(19), 10449.
markova s.v., larionova m.d., burakova l.p., vysotski e.s.
The smallest natural high-active luciferase: cloning and characterization of novel 16.5-kDa luciferase from copepod Metridia longa // Biochemical and biophysical research communications. – 2015. –V. 457. – P. 77-82.
Other laboratories and scientists
Hosting organization
Field of studies
Invited researcher
Time span of the project
Laboratory for Super-elastic Bio-interfaces

Tomsk State University - (TSU)

Medical biotechnologies


Volinsky Alexei Aleksandrovich

USA, Russia


Laboratory for Omics Technologies and big data for Personalized Medicine and Health

Skolkovo Institute of Science and Technology - (Skoltech)

Medical biotechnologies


Borchers Christoph Hermann


Laboratory for Molecular Imaging

Federal State Institution «Federal Research Centre «Fundamentals of Biotechnology» of the RAS» - (Research Center of Biotechnology RAS)

Medical biotechnologies


Bogdanov Alexey Alexeyevich

United States, Russia

Zherdeva Vitoriya Vyacheslavovna