Laboratory for Pharmacological Regulation of Cell Resistance

A new species was shown and the main mechanisms of multicellular resistivity of leukemic cells were investigated. The mechanism of increasing the resistance of cells of acute myeloid leukemia to death induced by DNA-tropic compounds, based on the activation of adhesion to the main molecules of the extracellular matrix, has been investigated. A new mitochondria-oriented mechanism of the antitumor action of melatonin has been shown. A new mechanism of astaxanthin cardioprotective action has been shown. A new mechanism of mTPP regulation has been investigated due to the participation of cytosolic NADH and NAD, which are potent inhibitors of mTPP in differentiated cells. It has been shown that a high-salt diet leads to the development of hypertensive nephrosclerosis, due to a decrease in oxygen consumption, suppression of the renal antioxidant system and an increase in oxidative stress in the renal cortical glomeruli.

Implemented results of research:

The method for personalized screening of drug action on leukemic cells ex vivo.

It is used on the basis of the Department of Hematology and Immunotherapy of the Moscow Regional Research Clinical Institute named after V.I. MF Vladimirsky ”, to predict the success of personalized therapy for acute leukemia.

Education and career development:

Educational courses:

1. "Experimental Biomedicine" (Lomonosov Moscow State University, Faculty of Biotechnology, bachelor's degree).
2. "Biotechnology of animal and human cells" (Lomonosov Moscow State University, Faculty of Biotechnology, Master's degree).
3. "Pharmaceutical screening of drugs and promising substances in vitro" (PushGENI, Faculty of Biophysics and Biomedicine, Master's degree).

Teaching aids:

1. Akatov V.S., Fadeev R.S., Fadeeva I.S., Shatalin Yu.V., Fesenko N.I. Pharmaceutical screening of drugs and promising substances in vitro. 2014. - Publ .: "FixPrint", Pushchino, 62 pages, ISBN 978-5-903789-21-4.
2. Fadeeva I.S., Fadeev R.S., Senotov A.S., Akatov V.S. In vivo bioscreening of materials, preparations and promising substances. 2016. - Ed. SynchroBook, Pushchino, 59 pages, ISBN 978-5-91874-042-2.

Work shops:

1. "ADVANCED DRUG DESIGN TECHNOLOGIES: MULTI-PARAMETER HCS SCREENING FOR INNOVATIVE DRUGS SEARCH" (2014).
2. "THE BASIS OF 2D-ELECTROPHORESIS" (2015-2016).
3. "BIOIMAGING MICROSCOPY" (2015).
4. “SEMINAR ON PURIFICATION AND SEPARATION OF PROTEINS. TGX STAIN FREE TECHNOLOGIES "(2016).
5. " xMAP AND GENE EXPRESSION ANALYSIS" (2016).
6. "ORGANIZATION OF BEHAVIORAL EXPERIMENTS ON LABORATORY ANIMALS: VALIDATION, WORLD STANDARDS" (2016).
7. "DETERMINATION OF CELL VIABILITY BY METHODS OF FLOW CYTOMETRY" (2017).
8. "MODERN TECHNOLOGIES OF CELLULAR IMAGING" (2017).
9. " PCR SCHOOL " (2017).

Training of highly qualified personnel:

Nikiforova Anna Borisovna, PhD in Biology, 2015

Kharechkina Ekaterina Sergeevna, PhD in Biology, 2021

Moscow Regional Clinical Research Institute named after M.F. Vladimirsky (Russia), Lomonosov Moscow State University (Russia), Institute of Immunology FMBA of Russia, Institute of Gene Biology Russian Academy of Sciences, Institute of Molecular Biology named after V.A. Engelhardt Russian Academy of Sciences, Belozersky Research Institute of Physical and Chemical Biology, Moscow State University (Russia), Medical University of South Carolina, Charleston, SC (USA), Aurora Health Care, Milwaukee (Wisconsin, USA): scientific cooperation.