International Laboratory for the Creation of Means of Prevention of Socially Significant Infenctions of Productive Animals

Name of the project:

To create means for the prevention of socially significant infections in productive animals on the basis of modern nutrigenomics methods.

Goals of project:

To developed means for the prevention of socially significant infections in productive animals, including probiotic microorganisms, with targeted antagonistic activity with respect to causative agents of the food zooanthroponotic infections Campylobacter jejuni, Salmonella enterica var. Enteritidis, Escherichia coli. The developed complex of probiotic agents will ensure the immune homeostasis, increase the security of cattle, the productivity, safety and quality of meat.

Project objective:

1. To create an innovative symbiotic consortium on the basis of the most promising candidate strains of probiotic microorganisms demonstrating targeted antagonistic activity with respect to a wide range of pathogenic E. coli and a prebiotic for the prevention of intestinal and extraintestinal infectious diseases caused by pathogenic antibiotic-resistant strains of E. coli, including globally widespread strains of a ST131 clone that produce ESBL in agricultural animals;
2. To study the influence of the symbiotic consortium on the intestinal microbiota of agricultural animals and its preventive effect against the background of the pathogenic antibiotic-resistant E. Coli stain of the ST 131 clone that produces ESBL;
3. To conduct a transcriptome analysis of the expression of genes in enterocytes of agricultural animals when they interact with symbiotic consortia of microorganisms aimed at the prevention of intestinal and extraintestinal infectious diseases caused by the pathogenic antibiotic-resistant strains E. coli, including widespread strains of the ST131 clone that produces ESBL;
4. To create am innovative symbiotic consortium on the basis of the most promising candidate strains of probiotic microorganisms that demonstrate targeted antagonistic activity against S. enterica var. Enteritidis for preventing salmonellosis in agricultural animals.
5. To study the influence of the symbiotic consortium as a feed supplement on the intestinal microbiota of agricultural animals and its preventive efficiency against the background of the S. Enteritidis pathogen;
6. To conduct a transcriptome analysis of the expression of genes in enterocytes of agricultural animals when they interact with the symbiotic consortium of microorganisms aimed at the prevention of salmonellosis;
7. To create an innovative symbiotic consortium based on the most promising candidate strains of probiotic microorganisms demonstrating targeted antagonistic activity against the C. Jejuni pathogen and a prebiotic for the prevention of campilobacteriosis in agricultural animals;
8. To study the influence of the symbiotic consortium on the intestinal microbiota in agricultural animals and its preventive efficiency against the background of the C. Jejuni pathogen;
9. To conduct a transcriptome analysis of the expression of genes in enterocytes of agricultural animals when they interact with the symbiotic consortium of microorganisms aimed at the prevention of campilobacteriosis;
10. Using bioinformatics methods, to conduct a modeling of the role of the intestinal microbiota of the host organism on the regulation of congenital and adaptive immunity for the formation of the immune homeostasis of the digestive system in the light of the development of the general theory of immunity.