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Contract number
Time span of the project

As of 01.11.2022

Number of staff members
scientific publications
Objects of intellectual property
General information

Name of the project: Biotechnology for the development of horticulture: propagation of healthy plant varieties and scaling up production

Goals and objectives

Project objective: Development of efficient methods for fast and bulk breeding and healing of economically important for horticulture in the setting of Crimea

The practical value of the study

Scientific results:

  1. Over the course of the implementation of the project, we have developed protocols for fast and efficient replication and healing of agricultural varieties in cultures in vitro for grapes (Vitis vinifera L.), blackberries (Rubus subgen. Rubus L.), raspberries (Rubus idaeus L.), strawberry (Fragaria×ananassa (Duchesne ex Weston) Duchesne ex Rozier.), garlic (Allium sativum L.).
  2. For the first time on the territory of Russia, we have developed a bioreactor technology for the replication of grape and strawberry plants. Over the six weeks of the experiment, the biomass of the plants increased by a factor of 300, the replication rate of strawberry compared to the control sample was 20 times higher, for grapes it was 5 times higher. We are now refining the protocol for other cultures.
  3. We have developed methods for molecular genetic analysis based on determining variety-specific markers to control varietal purity and genetic stability of replicated plants. We are working to create a modern test system for prompt diagnostics and identification of pathogens for plant varieties of Southern Russia that are economically significant in gardening.
  4. The Laboratory is creating an elite breed shed of the researched variety of strawberry, which will be free of diseases and will feature a system for controlling the virological status.

Implemented results of research:

The conducted applied research under the supervision of the leading scientist are relevant and effective. As of now, the Laboratory is fulfilling 8 contracts for the production of 529 000 plants of the research varieties using the tissue culture method for the needs of the agriculture. We are working to control the varietal purity of commercially valuable varieties, diagnostics and identification of pathogens.

Education and career development:

We have developed the professional development program «Basics of biotechnology and molecular diagnostics».

The Laboratory is implementing measures within the world-class inter-regional research and education center «Moreagrobiotech».

Organizational and infrastructural transformations:

  • On the grounds of the Laboratory, the Selection and Seed Production for Subtropical Fruit Crops. The Center participates in the implementation of the State program «Scientific and Technological Development of the Russian Federation», implementing the measures outlined in clause 4.6.4. «Creation, support and development of selection, seed production and breeding centers».
  • The Laboratory implements the agrobiotechnology at the Nizhniy Novgorod Center «Genetic and cell biotechnologies» as part of the federal project «Development of advanced infrastructure for conducting research and development in the Russian Federation» of the national project «Science».


Federal Research Center «Fundamentals of biotechnology» of the Russian Academy of Sciences, Mendeleev University of Chemical Technology of Russia, Lobachevskiy Nizhniy Novgorod State University (Russia): joint research.

N. I. Vavilov All-Russian Institute of Plant Genetic Resources (Russia): internships of employees.

The Laboratory collaborates with such companies as «Alma Valley Berries» Ltd, «Sweet strawberry» Ltd, «Crimean vegetable factory» Ltd, «Agro Prom» Ltd, «Invest plus» Ltd (Alma Valley), Agricultural production cooperative «Terruar» (UPPAWinery), Agricultural consumer processing cooperative «Bekovskiy Koopprodukt», «Agroindustrial Holding «Miratorg» (Russia).

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kryukov, l. a., vodolazhsky, d. i., & kamenetsky-goldstein, r.
(2022). Micropropagation of Grapevine and Strawberry from South Russia: Rapid Production and Genetic Uniformity. Agronomy, 12(2), 308
kamenetsky-goldstein, r., & yu, x.
(2022). Cut peony industry: the first 30 years of research and new horizons. Horticulture Research, 9.
khodakov, g. v., kryukov, l. a., shemesh-mayer, e., & kamenetsky-goldstein, r.
(2022). Biotechnology of virus eradication and plant vaccination in phytobiome context. Plant Biology, 24(1), 3-8.
oberemok, v. v., puzanova, y. v., kubyshkin, a. v., & kamenetsky-goldstein, r.
(2021). Variability in the Chemical Composition of a New Aromatic Plant Artemisia balchanorum in Southern Russia. Plants, 11(1), 6.
bhusal, h., shemesh-mayer, e., forer, i., kryukov, l., peters, r., & kamenetsky-goldstein, r.
(2021). Top Three Strategies of ss (+) RNA Plant Viruses: Great Opportunists and Ecosystem Tuners with a Small Genome. Viruses, 13(11), 2304.
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